LB Miller Broth - 2Kg
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LB Miller Broth - 2Kg LB Miller Broth - 2Kg

LB Miller Broth - 2Kg

  • $182.88 Model Number IB49030



  • Contains casein digest peptone, sodium chloride, and yeast extract
  • For maintaining and propogating E. coli culture

IBI’s Luria Broth (LB Miller Broth) is used in molecular genetic studies, as well as maintaining and propagating E. coli in molecular and microbiology procedures.  The inclusion of casein peptone and yeast extract provides essential growth factors for the bacteria; such as nitrogen, sulfur, minerals, and vitamins. Sodium chloride provides essential electrolytes.

To prepare, mix 25 g of the medium in 1 L of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Then, distribute and autoclave at 121.0⁰C for 15 minutes.

Store at room temperature, tightly sealed, and protect from moisture and light.

Physical Specifications
CAS#: N/A
pH (@ 25°C): 7.0 ± 0.2
NOTE: Grams per liter may be adjusted to obtain desired performance.

LB Miller Broth Specification Sheet

Formula per Liter
● Casein Digest Peptone 10.0 gm
● Sodium Chloride 10.0 gm
● Yeast Extract 5.0 gm

Preparation
● Mix 25 gm of the medium in 1 L of purified water until evenly dispersed.
● Heat with repeated stirring, and boil for one minute to dissolve completely.
● Distribute and autoclave at 121.0°C for 15 minutes.

Recommended Use
IBI’s Luria Broth (LB Miller) is used in molecular genetic studies, as well as maintaining and propagating E.coli in molecular and microbiology procedures. The inclusion of casein peptone and yeast extract supply essential growth factors; such as nitrogen, sulfur, minerals, and vitamins. Sodium chloride provides essential electrolytes.

Storage
Store at room temperature. Keep tightly sealed. Protect from moisture and light.

Warning
Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.