Kanamycin Sulfate - 25g
- For use in cell culture media to inhibit gram-positive and gram-negative bacteria
IBI Kanamycin is a broad spectrum aminoglycoside antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in cell culture media for the isolation of Group D Streptococci on Kanamycin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin medium. Kanamycin sulfate can also be used as selection agent for cells transformed with kanamycin B resistance gene.
IBI kanamycin acts by binding to the 70S ribosomal subunit, inhibiting translocation.
Kanamycin sulfate is effective against gram negative and gram positive bacteria, as well as mycoplasma.
A typical stock solution is 10mg/ml in water. Solution should sterilized via filtration through a 0.22 micron filter.
A working concentration for stringent plasmids is 10ug/ml and 50ug/ml for relaxed plasmids.
IBI Kanamycin sulfate is derived via microbial fermentation. No animal products or by-products are used in the manufacturing process or the fermentation media.
Product is packaged in a glass amber bottle and should be kept at 4 deg. C. This product ships on ice packs via UPS Blue Monday thru Wednesday. Orders placed after Noon on Wednesday are held until the following Monday.
Formula Weight: 582.58
Molecular Formula: C18H36N4O11 x H2SO4
Potency: >750 mcg/mg
pH (1%, water): 6.5 – 8.5
Moisture: Max. 4.0%
Molecular Biology Specifications
Antibiotic Inhibition/Sensitivity Assay: Pass
Chromatographic Purity: Pass
Kanamycin Sulfate is derived via microbial fermentation. No animal products or by-products are used in the manufacturing process or the fermentation media.