IBI Taq HotStart KeenGreen 2X Master Mix – 10 Rxns
- Contains KeenGreen loading dye (blue ~ 4Kb, yellow ~ ˂25bp)
- Applications: End-Point PCR, Colony/Whole Cell PCR, Sequencing Preparation, RT-PCR, Two-Step PCR, and Low-Copy Target
- Contaminant- and Nuclease-Free
IBI Taq HotStart KeenGreen 2X Master Mix is a premixed solution that suppresses enzymatic activity at room temperature. The master mix contains IBI Taq HotStart DNA Polymerase, a chemically modified enzyme with a near full recovery of the 5’ → 3’ polymerase and exonuclease activities following a 2-minute initial denaturation period.
IBI Taq HotStart KeenGreen 2X Master Mix contains reaction buffer, MgCl2, dNTPs, KeenGreen (blue and yellow) loading dye, and a density agent. The combination of the proprietary blue and yellow loading dyes and density agent allow for easy monitoring of electrophoresis progress and provide low inhibition of the PCR reaction. The dyes also prevent color smearing on a gel, do not interfere with band visualization, and are great for photos and publications. The KeenGreen PCR product can be added directly to an agarose gel.
1 x 250 µl – IBI Taq HotStart DNA Polymerase in 2X PCR Buffer (pH 8.5), KeenGreen dye, density agent, 400 µM dATP, 400 µM dCTP, 400 µM dGTP, 400 µM dTTP, 3 mM MgCl2