IBI Taq Hotstart DNA Polymerase w/ Reaction Buffer (Includes MgCl)- 100 Units
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IBI Taq Hotstart DNA Polymerase w/ Reaction Buffer (Includes MgCl)- 100 Units

  • $156.00 Model Number IB43221



  •  High-copy of PCR product at low concentrations of enzyme or DNA template
  • Superior enzyme stability and activity
  • Complete suppression of enzymatic activity at room temperature
  • Buffer can range from pH 6 to 10
  • Applications: End-Point PCR, Colony/Whole Cell PCR, RT-PCR, Two-Step PCR, Low-Copy Target, and Primer-Dimer Reduction

IBI Taq HotStart DNA Polymerase is a chemically modified hot start enzyme excellent for preventing or minimizing non-specific DNA amplification. The novel modifying reagent creates a faster activation time, an improvement over similar chemically modifed hot start enzymes. The alkaline activation condition makes for greater activity and better specific amplification which minimizes primer-dimers. The superior stability of IBI Taq HotStart also means longer shelf life!

Under standard hot start conditions (94C in Tris w/ pH 8-9) IBI Taq HotStart regains both the 5’ → 3’ polymerase and 5’ → 3’ exonuclease activity within two minutes. IBI Taq HotStart suppresses enzyme activity at room temperature with near full recovery of enzyme activity following activation. Our proprietary purification process also produces a contaminant- and nuclease-free enzyme that offers high-copy PCR products from low concentration template or enzyme.

Contents:

1 x 20 µl – IBI Taq HotStart DNA Polymerase

1 x 1 ml – PCR Buffer w/ 15 mM MgCl2*

1 x 1 ml – 25 mM MgCl2

*10X PCR Buffer contains: 100 mM Tris-HCl (pH 8.4), 500 mM KCl, 15 mM MgCl2