HEPES, Free Acid – 50 g
- For use in polyacrylamide electrophoresis buffer systems.
- Ideal cell culture buffer.
- Maintains physiological pH in cell culture applications.
HEPES powder is a zwitterionic biological buffer ideally suited for cell culture due to its ability to maintain physiological pH despite changes in carbon dioxide concentration. This property distinguishes HEPES from bicarbonate buffers which are also used in cell culture applications. Another characteristic of HEPES is that its disassociation decreases as temperature decreases making it a more effective buffer agent for maintaining enzyme structure and function at low temperatures.
Typical concentrations range from 10 to 25 mM. HEPES has a buffering range of pH 6.8 to 8.2.
HEPES is also a component of Tris-HEPES which is a buffer commonly used in polyacrylamide gel electrophoresis of proteins.
Offered in 50g, 250g, 500g and 1Kg sizes for your convenience.
Appearance: White Crystalline Powder
Molecular Formula: C8H18N2O4S
Molecular Weight: 238.31
pH (0.5 M solution in H2O): 5.1 to 6.5
Purity: Min. 99.0
Water: Max. 0.25%
Heavy Metals: Max. 0.001%
Iron: Max. 0.0005%
Sulfates: Max. 0.05%
Molecular Biology Specifications
DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
Cell Culture Testing: Pass