Half-Baffle TUNAIR Shake Flask Kit (Silicone), 2.5L
- Comes complete with four (4) polypropylene 2.5L Half-Baffle TUNAIR™ Shake Flasks, four (4) two-piece caps, and five (5) silicone filters.
The TUNAIR™ Shake Flask Systems are a unique and patented flask and closure system, designed for microbiology and biotechnology applications. This system provides optimum growth conditions for aerobic microorganisms, mammalian cells, and plant cells. They also provide better culture growth and productivity than standard Erlenmeyer flasks. The TUNAIR™’s high oxygen absorption rate is due to the unique baffling and turbo-vane closure design. The TUNAIR™ systems are designed to increase the availability of dissolved oxygen as well as improve cell yields.
The Half-Baffle Shake Flask has two baffles that produce a vortex motion. The working volume is 1L.
The silicone filters are 0.22 micron and will last indefinitely unless punctured. The silicone filters do not need to be washed, but can be rinsed with water if visibly soiled. It is recommended to always leave the filter in the cap and autoclave.
The flasks are constructed of polypropylene and resistant to most solvents.
All TUNAIR™ flasks and caps can be cleaned by soaking in water with a light detergent solution to loosen dirt and contaminants, then air dry.
Flask Size: 2.5L
Working Volume: 1L
Base Diameter: 6.50” [16.51cm]
Neck Diameter: 3.00” [7.62cm]
Height: 10.75” [27.31cm]
Weight: 0.02 lbs. [0.008Kg]
Half-Baffle (2 Baffles): Vortex Motion
1” Throws: 300-400rpm or possibly higher
2” Throws: 150-200rpm or possibly higher
All TUNAIR flasks and caps are constructed of chemical resistant polypropylene. All flasks and caps are fully autoclavable.
All TUNAIR flasks and caps can be cleaned by soaking in water with a light detergent (i.e. Joy) solution to loosen dirt and contaminants; air dry. All TUNAIR flasks and caps can be autoclaved, if required.
Cell Growth Evaluation of Commonly Used Shake Flasks
TUNAIR™ flasks were compared to conventional flasks using four different types of microorganisms; Escherichia coli, Saccharomyces cerevisiae, Penicillium avellaneum, and Streptomyces chartreusis. The aeration capacities of the shake flasks were determined by the sulfate oxidation method, and the values shown below are presented as oxygen absorption rate (OAR) in mM oxygen/L/Min. The growth rates of E.coli and S.cerevisiae were expressed as optical densities (OD) at 555mM. For S.chartreusis and P.avellaneum growth rates were evaluated by percent sedimentation. For E.coli and S.cerevisiae, the growth rates were determined after an 18-hour incubation period; for S.charteusis, a 24-hour incubation period; and for P.avellaneum, a 72-hour incubation period. Growth and OAR evaluations were carried out with 3-9 replicates and statistically analyzed using Turkey’s w-procedure. See results below.
|OAR Value||OD @ 555mM||% Sedimentation|
|Triple Indented Flasks||2.47||5.97||5.31||19.20||9.50MP|
*Growth Morphology: M, mycelial; P, pellet; MP, mixed mycelial. The mycelial growths mostly adhered to the walls of the flask, which accounted for the low overall sedimentation value.