gMAX Mini Genomic DNA Kit – 300 Prep
- Sample Size: 50-200µl whole blood (fresh or frozen)/up to 25mg tissue/up to 25mg paraffin-embedded tissue/buccal swab/up to 15ml amniotic fluid/1×107 cultured cells/25mg insects/10 hairs (including follicle cells)
- Expectant Yield: up to 50µg DNA
- Operation Time: 20min or less
- Elution Volume: 30-100µl
- Free sample available upon request
The gMAX DNA Mini Kit has been optimized for genomic, mitochondrial, and viral DNA purification from whole blood (fresh and frozen) tissue, formalin-fixed paraffin-embedded tissue, cultured cells, buccal swab, amniotic fluid, hair, and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a wash buffer and the purified genomic DNA is eluted by a low salt elution buffer, TE, or water. The entire procedure can be completed in less than 30 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA is suitable for use in PCR or other enzymatic reactions.
The quality of the gMAX DNA Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 µl of whole human blood. The purified DNA (5 µg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
The gMax kit is one of the most versatile genomic DNA extraction kits on the market today. It has the ability to purify genomic DNA from a wide array of sample types with easy to use protocols and a very economical price point. If you wish to try a FREE sample of the gMax kit, please email us at firstname.lastname@example.org
- Technical Information
- Replacement Buffers
- Replacement Columns & Collection Tubes
|Specifications||gMax Mini Genomic DNA Kit|
|Binding Capacity:||50 µg|
|Sample Concentration:||200 µl blood 25 mg animal tissue 1x107 cultured cells hair|
|Sample Type:||Rodent tails, ear punches, fresh & frozen blood, plasma, serum, buffy coat, body fluids cultured cells, amniotic fluid, hair, insects, FFPE|
|Typical Yield:||5 µg from 200 µl blood/ 10-40 µg from 20 mg tissue|
|Elution Volume:||30- 100 µl|
|Operation Time:||<20 minutes|
What competitor’s kit is comparable? QIAGEN DNeasy kit
Can QIAGEN reagents be used with IBI columns? Yes, anything that is “lysis-based” will work with IBI columns.
Can I extract Genomic DNA from a Milk sample using this kit? Yes this kit will work when using a Milk sample. For small samples (200 µl) use the blood protocol. For larger samples (1 ml) use the amniotic fluid protocol.
When the tissue sample is slightly degraded, what can I do to improve the low yield of gDNA? 1. After adding the GT buffer and Pro K to the sample, proceed to vortex, and then incubate over night at 60°C. During the overnight incubation use an automated shaking water bath to continually agitate the sample during incubation. 2. Perform the RNA removal step as recommended in the protocol. 3. Pre-heat the elution buffer to 60°C for the elution step. 4. Using large amounts of elution buffer (100 µl – 200 µl) during the elution step can raise the DNA yield. 5. Homogenize tissue samples prior to lysis to help in the Pro-K digestion.
Will the gMax Kit work for fecal matter? You can purchase our IBI Fecal DNA kit for extracting DNA from fecal matter (IB47820-IB47822).
What is the typical tissue yields using the gMax kits? Depending on the starting material, it can be anywhere from upwards of 50µg with 25 mg of tissue, or between 10-40µg with 20 mg of tissue.
Where can I get more of the Proteinase K Solution? You can purchase our Proteinase K Solution-5 ml (IB05406 20 mg/ml) and dilute it with purified water (ddH20) to a concentration of 10 mg/ml.