Basic Agarose & 100 bp DNA Ladder Kit
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Basic Agarose & 100 bp DNA Ladder Kit Basic Agarose & 100 bp DNA Ladder Kit Basic Agarose & 100 bp DNA Ladder Kit

Basic Agarose & 100 bp DNA Ladder Kit

  • $395.00 Model Number IB01320



Basic Agarose (CAS#: 9012-36-6)

  • Certified DNase- and RNase-Free for standard electrophoresis procedures
  • Excellent gel strength and clarity

100 bp DNA Ladder

  • Ready-to-use DNA Ladder (pre-mixed with loading dye)
  • Suitable for sizing double-stranded DNA from 100 bp to 3 Kb with enhanced intensity at 500 bp
  • Concentration: 50µg / 500µl; (Recommended loading: 0.5ug or 5ul)

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Basic Agarose

IBI Basic Agarose is ideal for standard electrophoresis procedures such as, rapid separation of DNA and RNA fragments, PCR products, preparation of plasmids and for screening, cloning and blotting techniques.

IBI Basic Agarose is a neutral polysaccharide extracted from the cell walls of certain Rhodophyceae which are also known as agarophyte seaweeds. The structure of the polysaccharide is a galactan, formed by linking agarobioses 1-3, 1-4. This chemical structure gives agarose the capacity to form gels that are very strong even at low concentrations. These gels have a macroreticular structure with a very open mesh which can be adjusted simply by varying the concentration of agarose.

IBI agarose is molecular biology certified and refined for use in every day routine electrophoresis applications. IBI Basic agarose provides a more economical choice for procedures that do not involve resolving small (<1000 bp) DNA fragments or fragment that are within 15% to 20% in size of each other.

100 bp DNA Ladder

The IBI 100bp DNA Ladder, containing 12 linear double-stranded DNA fragments, is suitable for sizing double-stranded DNA from 100bp to 3,000bp. The 100bp DNA Ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 12 fragments, suitable for use as molecular weight standards for electrophoresis. The enhanced intensity of the 500bp fragment was designed for quick and easy determination of electrophoresis results.

Physical Specifications

Basic Agarose:
CAS#: 9012-36-6
Ash: ≤ 4.50%
Sulfate: ≤ 0.10%
Clarity @ 1.5% (NTU): ≤ 4 NTU
Gel Strength @ 1.0% (g/cm2): ≥ 1000
Gel Strength @ 1.5% (g/cm2): ≥ 2200
Gelling Temperature @ 1.5%: 36°C ± 1.5°C
Melting Temperature @ 1.5%: 88°C ± 1.5°C

Molecular Biology Specifications
DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
Endonuclease assay: None Detected
Gel analysis assay: Pass

IBI Basic Agarose - Specification Sheet

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100 bp DNA Ladder
Product Size: 50µg Volume: 500µl Concentration: 100µg/ml Recommended Loading: 0.5µg (5µl) Number of Gel Lanes: 100 applications Size Range: 100bp to 3,000bp Number of Bands: 12 Bands with Enhanced Intensity: 500bp Ready to Load: Yes Gel Compatibility: Agarose gels Tracking dyes: Bromophenol blue, xylene cyanol FF Storage Conditions: 25°C (6 months), 4°C (12 months), -20°C (24 months) Shipping Conditions: Approved for shipping at room temperature or dry ice

Components: • 100 bp DNA ladder (50µg, 100µg/ml, pre-mixed loading dye, ready-to-use) • 6X DNA loading dye for sample loading

100 bp DNA Ladder - Specification Sheet & Protocol