96 Well Genomic DNA Bacteria Kit – 4 x 96 Well
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96 Well Genomic DNA Bacteria Kit – 4 x 96 Well

  • $1,366.29 Model Number IB47296



High-throughput, high yield, high purity genomic and viral DNA extraction from bacteria

  • High-throughput genomic and viral DNA extraction from Gram (+) positive and Gram (-) negative bacterial cells
  • Yields up to 5 µg of highly pure genomic DNA per well
  • 96 Well gDNA Binding Plates contain a glass fiber membrane optimized for genomic DNA extraction and sterilized to remove bacteria contamination
  • Includes a Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria

The 96 Well Genomic DNA Bacteria Kit is optimized for high-throughput genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells.  Gram+ Buffer, when combined with Lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer.  Proteinase K and chaotropic salt are used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the binding plate.  Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water.  Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.

The quality of the 96 Well Genomic DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (5 x 108) culture.  5 µl from a 100 µl eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.

 

Sample:  up to 5 x 108 Gram (+) positive and Gram (-) negative bacteria cells

DNA Size:  20 – 40 Kb

Yield:  up to 5 µg of genomic DNA per well

Format:  96 Well gDNA Binding Plate (glass fiber membrane)

Operation Time:  within 90 minutes

Elution Volume:  100 - 200 µl

Purity:  A260/A280 > 1.9

Kit Storage:  dry at room temperature (15 – 25°C); Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods