5X RNA loading Dye
5X RNA loading Dye
Model Number IB01015
- Item Description
- Physical Specifications
- Certificate of Analysis
- SDS
- Technical Information
- Used in RNA electrophoresis when using a formaldehyde gel and MOPS buffer
The 5X RNA Loading Dye Kit contains reagents for denaturing and loading RNA samples onto a formaldehyde gel and using MOPS as a buffer. Reagents are nuclease free and contain ultra-pure deionized formamide and DEPC water.
To use, the RNA sample is dissolved in 10 µl of DEPC water and mixed with 35 µl of denaturing solution. Heat the sample to 65⁰C for 5 minutes. Once the solution has cooled, add 5 µl of loading dye. The sample is now ready to load into the agarose or formaldehyde gel.
CAS#:N/A
Molecular Formula:Not Applicable
Molecular Weight:Not Applicable
Molecular Biology Specifications
DNase assay: None Detected
RNase assay: None Detected
Recommended Use
The 5X Gel Loading Kit contains reagents for denaturing and loading RNA samples onto a formaldehyde gel using MOPS as a buffer. The reagents are nuclease free and contain ultra pure deionized formamide and DEPC water.
The RNA sample is dissolved in 10 μl of DEPC water mixed with 35 μl of denaturing solution. Heat the sample to 65ºC for 5 minutes. Once the solution has cooled, add 5 μl of loading dye. The sample is now ready to load into the gel.
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