10X TAE Buffer Concentrate - 1L
- Electrophoresis buffer containing Tris, Acetate, and EDTA
TAE (Tris / Acetate / EDTA) buffer is commonly used in low voltage DNA electrophoresis applications. It’s relatively low buffering capacity will become exhausted in extended or current electrophoresis runs making buffer recirculation necessary in such runs. Potential advantages of TAE over TBE buffer include superior resolution of supercoiled DNA and slightly faster migration of double stranded DNA fragments.
10 X TAE Buffer Concentrate will produce 10 L of 1 X solution, but smaller quantities can be produced.
- 0.4 M Tris-Acetate
- 0.01 M EDTA
pH (1 x solution @ 25°C): 8.3 ± 0.1
Molecular Biology Specifications
Gel Analysis assay: Pass