10 x 96 Well Plasmid Kit
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10 x 96 Well Plasmid Kit

  • $1,098.08 Model Number IB47155



  • 10 x 96 Well Plasmid Kit
  • Centrifuge or vacuum format
  • 1 – 5 ml cultured bacterial cells
  • Filter plates, deep well plates, and collection plates included

The 10 x 96 Well Plasmid Kit was designed for high-throughput purification of plasmid DNA from 1 – 5ml of cultured bacterial cells per well. The kit utilizes a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. This kit requires a centrifuge or vacuum manifold capable of running 96 well plates. IBI offers IB47500 96 well vacuum manifold.

Typical yields are 20 – 35ug for high-copy number plasmid and 3 – 10ug for low-copy number plasmid from 4ml of cultured bacterial cells (E. coli). The purified DNA is ready for use in restriction enzyme digestion, ligation, PCR and sequencing reactions. Click on the tabs above to review the protocol or detailed specifications.

 

Specifications 96-Well Plasmid Kit Component 10 x 96 Well
Format: 96-Well Plate PD1 Buffer1: 200 ml
Binding Capacity: 35 µg PD2 Buffer2: 200 ml
Culture Input: 1-5 ml PD3 Buffer: 60 ml x 1 240 ml x 1
Culture Type: Bacteria culture W1 Buffer: 200 ml x 2
Plasmid Size: 1-15 kb Wash Buffer3: (Add Ethanol) 25 ml x 1 (100 ml x 1) 50 ml x 2 (200 ml x 2)
Typical Yield: 20-35 µg/ well Elution Buffer: 120 ml
Elution Volume: 100 µl RNase A: (50 mg/ ml) 650 µl
Operation Time: 25 minutes Plasmid 96 Well Filter Plate: 10 (IB47152)
Plasmid 96 Well Binding Plate: 10
96 Deep Well Plate: 10 (IB47155)
0.35 ml Collection Plate: 10
Adhesive Film: 40