The rYeast Total RNA Mini Kit was designed for total RNA purification from yeast and a wide variety of other fungus species. Sorbitol Buffer is included with the kit to reduce sample preparation time and minimize hands on time. Detergents and chaotropic salt are used to lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free water. The entire procedure can be completed within 70 minutes and the purified RNA is ready-for-use in RT-PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis.
The quality of the rYeast Total RNA Mini Kit is tested on a lot-to-lot basis by isolating RNA from Saccharomyces cerevisiae (5×107) harvested by centrifugation at 5,000xg for 10 minutes. A 5µl aliquot of purified RNA from a 50µl eluate is analyzed by electrophoresis on a 0.8% agarose gel.
• Sample Size: up to 5×107 fungus cells • Expected Yield: up to 30µg of RNA • Elution Volume: 50-100µl • Operation Time: within 70 minutes