RNA Isolation from Bacteria

IBI offers two different kits for extracting RNA from gram positive and gram negative bacteria.

Reference the differences below between the rBac Total RNA Kit and the Isolate DNA/RNA/Protein Reagent Kit:

• rBac Total RNA Kit: spin column, 30 minute operation time, 1×109 bacterial cells sample size, expected yield up to 60µg RNA. No use of phenol or guanidine isothiocyanate.

• Isolate DNA/RNA/Protein Reagent Kit: reagent based extraction, 60 minute operation time, scalable sample size. Uses phenol, chloroform, and guanidine isothiocyanate for RNA extraction.