IBI’s MP-1015 Multi-Purpose Gel Box (10 x 15 cm) is a general purpose unit for both extended high-resolution linear runs as well as economical quick screens. It is equipped with recirculation ports for procedures requiring buffer recirculation. The MP-1015 is constructed of durable cast acrylic, which will withstand high voltages and extreme temperatures without cracking or warping, unlike most molded units. The MP-1015 can be transformed into a quick screening unit with samples resolved in 30-60 minutes. Resolution of restriction digestions, plasmid purification, and ligation reactions are all possible with short runs. The MP-1015 is also compatible with a number of analytical and preparative combs for every horizontal electrophoresis application.
The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the MP-1015 is 140V. In a 1% TBE gel this translates into a run time of approximately one hour. Lower voltages may be used and as a general rule a 70 V run will take twice as long as a 140V run. Higher voltages may be used to decrease run time. However, if the unit is being operated at higher voltages than 140 V the heat generated during electrophoresis may decrease sample resolution. Such artifacts may be avoided by running the unit in a cold room, recirculating buffer, or adding 1X electrophoresis buffer “ice cubes” to keep the unit properly cooled. The suggested run parameters for the electrophoresis of RNA in agarose gels containing formaldehyde is 60-80 V.
The MP-1015 Electrophoresis System (IB53000) comes with: vented lid, UVT casting tray, casting fixture, two 2.0 mm x 16 tooth combs, bubble level, buffer ports, adjustable feet, and power cords.
The MP-1015 Multi-Purpose DNA Start-Up Kit (IB53000B) comes with: MP-1015 Electrophoresis System, 5 ml of 6 X Loading Dye, 1 L of 10 X TAE Buffer, 1 L of 10 X TBE Buffer, and 100 g of Molecular Biology Grade Agarose.
• Gel Dimensions: 10.0 cm x 15.0 cm • Maximum Sample Capacity: 80 samples (4 combs, 20 samples ea) • Buffer Capacity: 500 ml • Distance Between Electrodes: 27.0 cm • Maximum Voltage: 200 V