The Select-D, G-25 microcentrifuge spin columns are intended for use in desalting, recovering DNA fragments (>12mer), and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from small volume 5’ end-labeling reactions and fill-in labeling reactions utilizing a DNA polymerase. In addition, the Select-D, G-25 spin columns are RNase-free and can be used for rapid purification of oligoribonucleotides and RNA from unincorporated ribonucleotides (rNTPs) and in other related applications. After a brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume.
Each DNase- and RNase-free column is prepackaged with Sephadex G-25 in sterile TE or STE buffer. Two nuclease-free collection tubes (autoclavable) are supplied for each column.