CAS# 6226-79-5

• Used to visualize proteins on a nitrocellulose or PVDF membrane

Ponceau S protein stain is used to visualize proteins on a nitrocellulose or PVDF membrane.

To create a Ponceau S solution, dissolve 0.5g of Ponceau S in 1ml of glacial acetic acid and bring to 100ml with ddi water.

To stain with Ponceau S, remove blot and rinse with ddi water. Then, stain with Ponceau S solution for 1 – 2 minutes. Rinse for 3 – 5 minutes with ddi water. Red bands should appear against the white background.

To reversal stain with Ponceau S solution, place the nitrocellulose membrane in Ponceau S solution for 5 minutes at room temperature. Destain in ddi water for 2 minutes and mark the molecular weight standard with indelible ink. To complete the destain, soak in water for 10 minutes. Alternatively, use 0.1M NaOH solution for 5 minutes if proteins of interest are not basic.

CAS#:6226-79-5
Molecular Weight:760.61
Molecular Formula:C22H12N4Na4O13S4
Em (520nm, water):> 27000
Loss on drying %:< 6%

Molecular Biology Specifications
RNase:None Detected
DNase:None Detected
Protease:None Detected

IB01100 - Specification Sheet

CofA IB01100 - Lot#18B0156

CofA IB01100 - Lot#16H0150

CofA IB01100 - Lot#13H1750

CofA IB01100 - Lot#14J3050

CofA IB01100- Lot#19C0158

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IB01100 - SDS

Recommended Use
Ponceau S is a stain used to visualize protein on nitrocellulose or PVDF membranes.