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| IBI Purification Products | |
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| IBI Purification Products - Hi-Speed Mini Plasmid Kit | |
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The IBI High-Speed Plasmid Mini kit is designed for the rapid isolation of plasmid or cosmid DNA from 1 - 4ml of bacterial cultures. The modified alkaline lysis method and RNAse treatment are used to create cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate, binds to the glass-fiber matrix of the spin column. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol extraction or alcohol preceipitation. Typical yields are 20-30ug of DNA for high-copy number plasmid or 3-10ug for low-copy plasmid |
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Sample: 1-4ml of bacterial culture Format: Spin column Operation: Centrifuge or vaccum manifold Binding Capacity: Up to 30ug Expected Yield: 20-30ug for high-copy plasmid 3-10ug for low-copy plasmid Operation time: 30 minutes Application: Fluorescent or Radioactive sequencing Restriction Digestion, Library screening Ligation and transformation. Click on Details, then click on Specs to view protocol. |
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Sample: 1-4ml of bacterial culture Format: Spin column Operation: Centrifuge or vaccum manifold Binding Capacity: Up to 30ug Expected Yield: 20-30ug for high-copy plasmid 3-10ug for low-copy plasmid Operation time: 30 minutes Application: Fluorescent or Radioactive sequencing Restriction Digestion, Library screening Ligation and transformation. Click on Details, then click on Specs to view protocol. Take advantage of this special price by referencing this code on your next purchase order. webDec08. |
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Sample: 1-4ml of bacterial culture Format: Spin column Operation: Centrifuge or vaccum manifold Binding Capacity: Up to 30ug Expected Yield: 20-30ug for high-copy plasmid 3-10ug for low-copy plasmid Operation time: 30 minutes Application: Fluorescent or Radioactive sequencing Restriction Digestion, Library screening Ligation and transformation. Click on Details, then click on Specs to view protocol. |
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| IBI Purification Products - High-Throughput Mini Plasmid Kit - 96 Well | |
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The 96-well Plasmid kit is designed for high-throughput plasmid or cosmid DNA from 1-2ml bacterial cultures. Binding washing and elution steps can be done using a vacuum manifold or by centrifugation. |
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Sample: 1-2ml bacterial culture Format: 96-well plates Operation: Centrifuge / Vaccuum Manifold Binding Capacity: 10ug per well Expectant Yield: 5-10ug for high-copy plasmid 0.5-5ug for low-copy plasmid Operation time: 30 minutes Applications: Fluorescent or radioactive sequencing restriction digestion, library screening ligation and transformation. Click on Details, then click on Specs to view protocol. |
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Sample: 1-2ml bacterial culture Format: 96-well plates Operation: Centrifuge / Vaccuum Manifold Binding Capacity: 10ug per well Expectant Yield: 5-10ug for high-copy plasmid 0.5-5ug for low-copy plasmid Operation time: 30 minutes Applications: Fluorescent or radioactive sequencing restriction digestion, library screening ligation and transformation. Click on Details, then click on Specs to view protocol. |
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Sample: 1-2ml bacterial culture Format: 96-well plates Operation: Centrifuge / Vaccuum Manifold Binding Capacity: 10ug per well Expectant Yield: 5-10ug for high-copy plasmid 0.5-5ug for low-copy plasmid Operation time: 30 minutes Applications: Fluorescent or radioactive sequencing restriction digestion, library screening ligation and transformation. Click on details, then click on Specs to view protocol. |
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| IBI Purification Products - Fast-Ion Midi Plasmid Kit | |
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IBI Plasmid MIDI kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Using a gravity flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be removed with wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isoporpanol for desalting. The entire process can be completed in less than 2 hours without ultracentrifuges or HPLC and other hazardous reagents. |
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Sample Sizes: High Copy Plasmid - 20-50ml Low Copy Plasmid - 50-150ml Format: Ion-exchange resin column Operation: Gravity flow Binding Capacity: 500ug/1mg Purity: Equal to that obtained by 2xCsCl-gradient centrifugation Operation time: 120 minutes Applications: Transfection, Sequencing, In Vitro Transcription Microinjection, Restriction Digestion. Click on Details, then click on Specs to view protocol. |
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Sample Sizes: High Copy Plasmid - 20-50ml Low Copy Plasmid - 50-150ml Format: Ion-exchange resin column Operation: Gravity flow Binding Capacity: 500ug/1mg Purity: Equal to that obtained by 2xCsCl-gradient centrifugation Operation time: 120 minutes Applications: Transfection, Sequencing, In Vitro Transcription Microinjection, Restriction Digestion. Click on Details, then click on Specs to view protocol. |
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| IBI Purification Products - Fast-Ion Midi Plasmid kit (Endotoxin Free) | |
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Endotoxins, are cell membrane components of Gram-negative bacteria such as E.coli. Endotoxins are released during the lysis step of plasmid purification and reduce transfection efficiencies in endotoxin-sensitive cell lines. Endotoxions represent a non-controllable variable in transfection experiment set-up Making results difficult to compare and interpret. IBI Fast-Ion Endo-Free MIDI Plasmid kits are designed to deliver clear cell lysate with minimal genomic DNA or RNA contaminants. The pre-packed anion exchange resin columns will purify DNA from 50 - 100ml of bacterial cultures. |
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The IBI Endo-Free MIDI Fast-Ion plasmid sample kit Quantity - 2 preps Sample size - Low Copy Plasmids = 25-50ml bacterial culture Sample size - High Copy Plasmids = 100-200ml of bacterial culture Yield - up to 200ug of plasmid DNA Format - Gravity flow Time - 120 minutes To view or download protocol: Click on Details, then click on specs. |
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The IBI Endo-Free MIDI Fast-Ion plasmid kit Quantity - 25 preps Sample size - Low Copy Plasmids = 25-50ml bacterial culture Sample size - High Copy Plasmids = 100-200ml of bacterial culture Yield - up to 200ug of plasmid DNA Format - Gravity flow Time - 120 minutes To view or download protocol: Click on Details, then click on specs. |
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| IBI Purification Products - Fast-Ion Maxi Plasmid Kit | |
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IBI Plasmid MAXI kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Using a gravity flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be removed with wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isoporpanol for desalting. The entire process can be completed in less than 2 hours without ultracentrifuges or HPLC and other hazardous reagents. |
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Sample: High-Copy Plasmid - 50 - 150ml Low-Copy Plasmid - 150 - 300ml Format: Ion-exchange Columns Operation: Gravity Flow Binding Capacity: 500ug / 1mg Purity: Equal to that obtained by 2xCsCl-gradient centrifugation Operation time: 120 minutes Applications: Transfection, Sequencing, In Vitro Transcription, Microinjection, Restriction Digestion Click on Details, then click on Specs to view protocol. |
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Sample: High-Copy Plasmid - 50 - 150ml Low-Copy Plasmid - 150 - 300ml Format: Ion-exchange Columns Operation: Gravity Flow Binding Capacity: 500ug / 1mg Purity: Equal to that obtained by 2xCsCl-gradient centrifugation Operation time: 120 minutes Applications: Transfection, Sequencing, In Vitro Transcription, Microinjection, Restriction Digestion Click on Details, then click on Specs to view protocol. |
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Sample: High-Copy Plasmid - 50 - 150ml Low-Copy Plasmid - 150 - 300ml Format: Ion-exchange Columns Operation: Gravity Flow Binding Capacity: 500ug / 1mg Purity: Equal to that obtained by 2xCsCl-gradient centrifugation Operation time: 120 minutes Applications: Transfection, Sequencing, In Vitro Transcription, Microinjection, Restriction Digestion Click on Details, then click on Specs to view protocol. |
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| IBI Purification Products - Fast-Ion Maxi Plasmid Kit (Endotoxin Free) | |
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Endotoxins, are cell membrane components of Gram-negative bacteria such as E.coli. Endotoxins are released during the lysis step of plasmid purification and reduce transfection efficiencies in endotoxin-sensitive cell lines. Endotoxions represent a non-controllable variable in transfection experiment set-up Making results difficult to compare and interpret. IBI Fast-Ion Endo-Free MAXI Plasmid kits are designed to deliver clear cell lysate with minimal genomic DNA or RNA contaminants. The pre-packed anion exchange resin columns will purify DNA from 50 - 100ml of bacterial cultures. |
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IBI Endo-Free MAXI Plasmid sample kit Quantity - 2 preps Sample Size - Low Copy Plasmids = 100ml of bacterial culture Sample Size - High Copy Plasmids = 250ml of bacterial culture Yield - 500ug - 1mg of plasmid DNA Format - gravity flow Time - 120 minutes To view or download protocol: Click on details, then click on specs |
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IBI Endo-Free MAXI Plasmid kit Quantity - 10 preps Sample Size - Low Copy Plasmids = 100ml of bacterial culture Sample Size - High Copy Plasmids = 250ml of bacterial culture Yield - 500ug - 1mg of plasmid DNA Format - gravity flow Time - 120 minutes To view or download protocol: Click on details, then click on specs |
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IBI Endo-Free MAXI Plasmid kit Quantity - 25 preps Sample Size - Low Copy Plasmids = 100ml of bacterial culture Sample Size - High Copy Plasmids = 250ml of bacterial culture Yield - 500ug - 1mg of plasmid DNA Format - gravity flow Time - 120 minutes To view or download protocol: Click on details, then click on specs |
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| IBI Purification Products - Gel/PCR DNA Fragment Extraction Kit | |
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IBI Gel/PCR DNA Fragments Extraction kits are designed to recover or concentrate DNA fragments (50bp - 10kb) from an agarose gel or any other enzymatic reaction. This method uses a chaotropic salt, guanidine thyocianate to dissolve the agarose gel and denature the the enzymes. The DNA fragments in the Chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants the purified DNA fragments are eluted by a low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. |
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Sample: Up to 300mg agarose gel slice Up to 100ul PCR product or other enzymatic reaction Format: Spin Column Operation: Centrifuge/Vacuum manifold Binding Capacity: 10ug DNA Expectant Yield: 80-90% for gel extraction 90-95% for PCR clean-up Operation Time: 15 min for PCR clean-up 20 min for gel extraction Applications: PCR Fluorescent or Radioactive sequencing Restriction Digestion, DNA labeling, Ligation. Click on Details and then click on Specs to view protocol. |
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Sample: Up to 300mg agarose gel slice Up to 100ul PCR product or other enzymatic reaction Format: Spin Column Operation: Centrifuge/Vacuum manifold Binding Capacity: 10ug DNA Expectant Yield: 80-90% for gel extraction 90-95% for PCR clean-up Operation Time: 15 min for PCR clean-up 20 min for gel extraction Applications: PCR Fluorescent or Radioactive sequencing Restriction Digestion, DNA labeling, Ligation. Click on Details, then click on Specs to view protocol. Take advantage of this Special Price by referencing this code on your next purchase order. webDec08. |
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Sample: Up to 300mg agarose gel slice Up to 100ul PCR product or other enzymatic reaction Format: Spin Column Operation: Centrifuge/Vacuum manifold Binding Capacity: 10ug DNA Expectant Yield: 80-90% for gel extraction 90-95% for PCR clean-up Operation Time: 15 min for PCR clean-up 20 min for gel extraction Applications: PCR Fluorescent or Radioactive sequencing Restriction Digestion, DNA labeling, Ligation. Click on Details and then click on Specs to view protocol. |
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| IBI Purification Products - Gel/PCR DNA Extraction Kit - 96 Well | |
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The IBI 96-well PCR extraction kit is designed for high-throughput DNA fragment recovery from PCR or other enzymatic reaction. DNA binding, washing, and elution steps can be done using a vacuum manifold or centrifugation |
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Sample: Up to 50ul of PCR product or other enzymatic reaction Format: 96-well Plates Operation: Centrifuge / vacuum manifold DNA Size: 50bp - 10kb Binding Capacity: 10ug per cell Expectant Yield: 90-95% for PCR Clean-up Operation time: 30 min for PCR Click on Details and then click on Specs to view protocol. |
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Sample: Up to 50ul of PCR product or other enzymatic reaction Format: 96-well Plates Operation: Centrifuge / vacuum manifold DNA Size: 50bp - 10kb Binding Capacity: 10ug per cell Expectant Yield: 90-95% for PCR Clean-up Operation time: 30 min for PCR Click on Details and then click on Specs to view protocol. |
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| IBI Purification Products - Genomic DNA Mini Kit - (Blood/Cultured Cell) | |
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The IBI Genomic DNA kit is specially designed for purification of total DNA from Blood and cultured cell. This includes genomic, mitochondrial and viral DNA. All from different volumes of blood samples and cultured cells. In this procedure, RBC lysis buffer is used to remove non-nucleated red blood blood cells and reduce hemoglobin contamination. |
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Sample: Blood, cultured cells and other body fluids Sample size: up to 300ul / 2ml / Format: Spin columns Operation: Centrifuge or vaccuum Binding Capacity: 10ug per well Expected yield: 50-500ug of DNA Operation time: 30-60 minutes Sample kit contains 4 preps Click on Details, then click on Specs to view protocol. |
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Sample: Blood, cultured cells and other body fluids Sample size: up to 300ul / 2ml / Format: Spin columns Operation: Centrifuge or vaccuum Binding Capacity: 10ug per well Expected yield: 50-500ug of DNA Operation time: 30-60 minutes IB47201 contains 100 preps Click on Details, then click on Specs to view protocol. |
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Sample: Blood, cultured cells and other body fluids Sample size: up to 300ul / 2ml / Format: Spin columns Operation: Centrifuge or vaccuum Binding Capacity: 10ug per well Expected yield: 50-500ug of DNA Operation time: 30-60 minutes IB47202 contains 300 preps Click on Details, then click on Specs to view protocol. |
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| IBI Purification Products - Genomic DNA Maxi Kit - (Blood/Cultured Cell) | |
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The IBI Genomic DNA kit for blood and cultured cells is specially designed for purification of total DNA, including genomic, mitochondrial, and viral from different volumes of blood and cultured cells. In this procedure, RBC Lysis buffer is used to remove non-nucleated red blood cells and reduce hemoglobin contamination. |
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Sample: Frozen Blood and cultured cells. Sample size: 10ml of blood Format: Spin columns Operation: Centrifuge or vaccuum Binding Capacity: 10ug per well Expected yield: 100-300ug of DNA Operation time: 30-60 minutes IB47210 contains 10 preps To view protocol: Click on Details Then click on Specs |
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Sample: Frozen Blood and cultured cells. Sample size: 10ml of blood Format: Spin columns Operation: Centrifuge or vaccuum Binding Capacity: 10ug per well Expected yield: 100-300ug of DNA Operation time: 30-60 minutes Kit contains 2 preps. To view the protocol: Click on Details Then click on Specs. |
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| IBI Purification Products - Genomic DNA Mini Kit-(Tissue/Blood/Cultured Cell) | |
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IBI Genomic DNA kits for tissue are specially designed for purification of total DNA including genomic, mitochondrial and viral DNA, from a variety of animal tissues or cells Provided micropestle can efficiently homogenize tissue samples to shorten the time of the lysis step. This method uses proteinase K and chaotropic salt to lyse cells and degrade protein, then DNA in chaotropic salt is bound to the glass fiber matrix of the column. After washing off the contaminants the purified DNA is eluted by a low salt elution buffer or water. |
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Sample: Animal tissues, mouse tails, swabs, blood and body fluids. Sample Size: up to 50mg of tissue or 200ul of blood. Format: Spin columns Operation: Centrifuge or vacuum Binding Capacity: 50ug Operation Time: 30 - 60 minutes Applications: PCR, AFLP/PADP, RFLP, Southern Blotting Real-time PCR Click on Details, then click on Specs to view protocol. |
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Sample: Animal tissues, mouse tails, swabs, blood and body fluids. Sample Size: up to 50mg of tissue or 200ul of blood. Format: Spin columns Operation: Centrifuge or vacuum Binding Capacity: 50ug Operation Time: 30 - 60 minutes Applications: PCR, AFLP/PADP, RFLP, Southern Blotting Real-time PCR Click on Details, then click on Specs to view protocol. |
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Sample: Animal tissues, mouse tails, swabs, blood and body fluids. Sample Size: up to 50mg of tissue or 200ul of blood. Format: Spin columns Operation: Centrifuge or vacuum Binding Capacity: 50ug Operation Time: 30 - 60 minutes Applications: PCR, AFLP/PADP, RFLP, Southern Blotting Real-time PCR Click on Details, then click on Specs to view protocol. |
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| IBI Purification Products - Genomic DNA Kit - (Plant) - 96 Well | |
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The 96-Well Genomic Plant DNA Kit provides an efficient method for isolating total DNA (genomic, mitochondrial and chloroplast DNA) from plant tissue and cells. Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. The unique GR Buffer is able to lyse most common plant samples and also samples high in polysaccharides. DNA phenol extraction is not required and the entire procedure can be completed in 1.5 hours. The isolated total DNA is ready for use in PCR, Real-time PCR, Southern Blotting, mapping and RFLP. |
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The 96-Well Genomic Plant DNA Kit provides an efficient method for isolating total DNA (genomic, mitochondrial and chloroplast DNA) from plant tissue and cells. Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. The unique GR Buffer is able to lyse most common plant samples and also samples high in polysaccharides. DNA phenol extraction is not required and the entire procedure can be completed in 1.5 hours. The isolated total DNA is ready for use in PCR, Real-time PCR, Southern Blotting, mapping and RFLP. |
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The 96-Well Genomic Plant DNA Kit provides an efficient method for isolating total DNA (genomic, mitochondrial and chloroplast DNA) from plant tissue and cells. Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. The unique GR Buffer is able to lyse most common plant samples and also samples high in polysaccharides. DNA phenol extraction is not required and the entire procedure can be completed in 1.5 hours. The isolated total DNA is ready for use in PCR, Real-time PCR, Southern Blotting, mapping and RFLP. |
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The 96-Well Genomic Plant DNA Kit provides an efficient method for isolating total DNA (genomic, mitochondrial and chloroplast DNA) from plant tissue and cells. Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. The unique GR Buffer is able to lyse most common plant samples and also samples high in polysaccharides. DNA phenol extraction is not required and the entire procedure can be completed in 1 hour. The purified genomic DNA is ready for use in PCR, Real-time PCR, Southern Blotting, mapping and RFLP. |
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The 96-Well Genomic Plant DNA Kit provides an efficient method for isolating total DNA (genomic, mitochondrial and chloroplast DNA) from plant tissue and cells. Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. The unique GR Buffer is able to lyse most common plant samples and also samples high in polysaccharides. DNA phenol extraction is not required and the entire procedure can be completed in 1 hour. The purified genomic DNA is ready for use in PCR, Real-time PCR, Southern Blotting, mapping and RFLP. |
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The 96-Well Genomic Plant DNA Kit provides an efficient method for isolating total DNA (genomic, mitochondrial and chloroplast DNA) from plant tissue and cells. Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. The unique GR Buffer is able to lyse most common plant samples and also samples high in polysaccharides. DNA phenol extraction is not required and the entire procedure can be completed in 1 hour. The purified genomic DNA is ready for use in PCR, Real-time PCR, Southern Blotting, mapping and RFLP. |
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| IBI Purification Products - Genomic DNA Mini Kit-(Blood/Cultured Cell)-96 Well | |
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The IBI 96-well Genomic DNA kits are deisgned for high-throughput purification of total DNA including mitochondrial, genomic and viral, from a variety of animal tissues and cells. Including: fresh blood, animal tissue, mouse tails swabs, cultured animal cells, cultured bacterial cells and cultured yeast cells. IBI 96-well Genomic DNA kits can be used for manual filtration or with robotic handling systems and the purified DNA, of approx. 20-30 kb is suitable for PCR or other enzymatic reactions |
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Sample: Fresh blood, animal tissue, mouse tails, swabs cultured animal cells, cultured bacterial cells, and cultured yeast cells. Sample size: 200ul of blood, up to 25mg of tissue 10-6 to 10-7 of cultured animal cells, less than 10-9 cultured bacterial cells, and 5x10-7 of yeast cells. Binding capacity: up to 30ug/well Format: 96-well plates Operation: centrifuge or vaccuum Applications: PCR, AFLP, RFLP, Southern blotting, rtPCR To view or download protocol: click on details, then click on specs. |
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Sample: Fresh blood, animal tissue, mouse tails, swabs cultured animal cells, cultured bacterial cells, and cultured yeast cells. Sample size: 200ul of blood, up to 25mg of tissue 10-6 to 10-7 of cultured animal cells, less than 10-9 cultured bacterial cells, and 5x10-7 of yeast cells. Binding capacity: up to 30ug/well Format: 96-well plates Operation: centrifuge or vaccuum Applications: PCR, AFLP, RFLP, Southern blotting, rtPCR To view or download protocol: click on details, then click on specs |
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Sample: Fresh blood, animal tissue, mouse tails, swabs cultured animal cells, cultured bacterial cells, and cultured yeast cells. Sample size: 200ul of blood, up to 25mg of tissue 10-6 to 10-7 of cultured animal cells, less than 10-9 cultured bacterial cells, and 5x10-7 of yeast cells. Binding capacity: up to 30ug/well Format: 96-well plates Operation: centrifuge or vaccuum Applications: PCR, AFLP, RFLP, Southern blotting, rtPCR To view or download protocol: click on details, then click on specs |
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| IBI Purification Products - Genomic DNA Mini Kit - (Plant) | |
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The IBI Genomic DNA Mini kits for plants provide a fast and simple method to isolate total DNA including, genomic, mitochondrial, chloroplast and viral from plant tissue and cells. Plant tissues are ground in liquid nitrogen and lysed by a buffer containing detergent. The tissue debris in lysate can be removed by the provided filter column. In the presence of chaotropic salt the genomic DNA in the lysate binds to the glass fiber matrix in the spin column. After washing off the contaminants, the purified DNA is eluted by a low salt buffer. |
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Sample: Plant tissues Sample Size: up to 100mg/1g of fresh sample. 20mg/200mg of dry sample. Format: Sipn column. Operation: Centrifuge/vacuum Binding Capacity: 50ug/500ug DNA Operation Time: 30 - 60 minutes. Click on Details, then click on Specs to view the protocol. |
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The IBI Genomic DNA Mini kit for plants provides a fast and simple method to isolate total DNA from plant tissue and cells. Including, genomic DNA, mitochondrial, chloroplast and viral DNA. Plant tissues are ground in liquid nitrogen and lysed by a buffer containing detergent. The tissue debris in lysate can be removed by the provided filter column. In the presence of a chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix in the spin column. After washing off the contaminants, the purified DNA is eluted by a low salt elution buffer or water Click on Details, then click on Specs to view the protocol. |
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| IBI Purification Products - Genomic DNA Maxi Kit - (Plant) | |
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The IBI Genomic DNA MAXI kits for plants provide a fast and simple method to isolate total DNA including, genomic, mitochondrial, chloroplast and viral from plant tissue and cells. Plant tissues are ground in liquid nitrogen and lysed by a buffer containing detergent. The tissue debris in lysate can be removed by the provided filter column. In the presence of chaotropic salt the genomic DNA in the lysate binds to the glass fiber matrix in the spin column. After washing off the contaminants, the purified DNA is eluted by a low salt buffer. |
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The IBI Genomic DNA Mini kit for plants provides a fast and simple method to isolate total DNA from plant tissue and cells. Including, genomic DNA, mitochondrial, chloroplast and viral DNA. Plant tissues are ground in liquid nitrogen and lysed by a buffer containing detergent. The tissue debris in lysate can be removed by the provided filter column. In the presence of a chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix in the spin column. After washing off the contaminants, the purified DNA is eluted by a low salt elution buffer or water Click on Details, then click on Specs to view the protocol. |
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The IBI Genomic DNA Mini kit for plants provides a fast and simple method to isolate total DNA from plant tissue and cells. Including, genomic DNA, mitochondrial, chloroplast and viral DNA. Plant tissues are ground in liquid nitrogen and lysed by a buffer containing detergent. The tissue debris in lysate can be removed by the provided filter column. In the presence of a chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix in the spin column. After washing off the contaminants, the purified DNA is eluted by a low salt elution buffer or water Click on Details, then click on Specs to view the protocol. |
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| IBI Purification Products - Total RNA Mini Kit - (Blood/Cultured Cell) | |
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IBI Total RNA Mini kits for Blood and Cultured Cells are specially designed for purification of Total RNA from cultured cells and fresh whole human blood. This method uses detergents and a chaotropic salt to lyse cells and inactivate RNase The RNA, in chaotropic salt, is bonded to the glass-fiber matrix of the column. After washing off contaminants, the purified RNA is eluted by RNase free water. |
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| IBI Purification Products - Total RNA Maxi Kit - (Blood/Cultured Cells) | |
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IBI Total RNA Maxi kits for Blood and Cultured Cells are specially designed for purification of Total RNA from cultured cells and fresh whole human blood. This method uses detergents and a chaotropic salt to lyse cells and inactivate RNase The RNA, in chaotropic salt, is bonded to the glass-fiber matrix of the column. After washing off contaminants, the purified RNA is eluted by RNase free water. |
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| IBI Purification Products - Total RNA Mini Kits - (Tissue/Blood/Cultured Cell) | |
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IBI Total RNA Mini kits for Tissue, Blood and Cultured Cells are specially designed for purification of Total RNA from a variety of animal tissue co-cells. The provided micro-pestle can efficiently homogenize tissue samples in the micro-centrifuge tube. This method uses detergents and chaotropic salt to lyse cells and inactivate RNase. The RNA, in the chaotropic salt, is bonded to the glass-fiber matrix of the column After washing off the contaminants, the purified RNA is eluted with RNase free water. |
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| IBI Purification Products - Total RNA Maxi Kit - (Tissue/Cultured Cell/Blood) | |
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| IBI Purification Products - Total RNA Mini Kit - (Plant) | |
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The IBI Total RNA Mini/Maxi kits for plants provides a fast and simple method to isolate total RNA from plant tissue and cells. In this process, the sample is ditributed by grinding in liquid nitrogen and then filtered by use of the filter column to remove cell debris. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to the glass fiber matrix in the spin column. The optional DNase treatments are used to remove DNA residues. The contaminants are are then washed with an ethanol based wash buffer Finally, the purified Total RNA is eluted by RNase free water. The protocl does not require phenol extraction or alcohol precipitation. |
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| IBI Purification Products - Total RNA Maxi Kit - (Plant) | |
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The IBI Total RNA Mini/Maxi kits for plants provides a fast and simple method to isolate total RNA from plant tissue and cells. In this process, the sample is ditributed by grinding in liquid nitrogen and then filtered by use of the filter column to remove cell debris. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to the glass fiber matrix in the spin column. The optional DNase treatments are used to remove DNA residues. The contaminants are are then washed with an ethanol based wash buffer Finally, the purified Total RNA is eluted by RNase free water. The protocl does not require phenol extraction or alcohol precipitation. |
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| IBI Purification Products - Total RNA Kit - (Animal Cultured Cells) - 96 Well | |
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| IBI Purification Products - miRNA Isolation kit | |
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IBI Micro RNA (miRNA) kits are designed for purification of micro RNA and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows for research into significant biological pathways for gene regulation. The standard protocol for isolating total RNA and mRNA ae not optimized for the isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA. In addition, the removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR and microarray analysis. This IBI kit is specially designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. |
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miRNA Isolation Sample kit Quantity - 4 preps Sample Size - 100mg of tissue / 1x10-6 cultured cells Time - 30 minutes To view or download protocol: Click on details, then click on specs. |
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miRNA Isolation Kit Quantity - 100 preps Sample Size - 100mg of tissue / 1x10-6 cultured cells Time - 30 minutes To view or download protocol: Click on details, then click on specs. |
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| IBI Purification Products - DNA Isolation Reagents | |
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IBI's RBC Lysis Buffer was designed specifically for DNA isolation from large volumes of blood. However, this highly versatile reagent is able to extract genomic DNA from various other samples, such as cultured cells, tissue, bacteria and fungus. RBC Lysis Buffer is included with the kit to remove non-nucleated red blood cells and to reduce hemoglobin contamination. When the blood sample is less than 50µl or when using nucleated red blood cell samples, the Cultured Cells Protocol is recommended. If a larger sample size is required, the buffer volume can be scaled proportionately. |
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IBI's RBC Lysis Buffer was designed specifically for DNA isolation from large volumes of blood. However, this highly versatile reagent is able to extract genomic DNA from various other samples, such as cultured cells, tissue, bacteria and fungus. RBC Lysis Buffer is included with the kit to remove non-nucleated red blood cells and to reduce hemoglobin contamination. When the blood sample is less than 50µl or when using nucleated red blood cell samples, the Cultured Cells Protocol is recommended. If a larger sample size is required, the buffer volume can be scaled proportionately. |
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IBI's RBC Lysis Buffer was designed specifically for DNA isolation from large volumes of blood. However, this highly versatile reagent is able to extract genomic DNA from various other samples, such as cultured cells, tissue, bacteria and fungus. RBC Lysis Buffer is included with the kit to remove non-nucleated red blood cells and to reduce hemoglobin contamination. When the blood sample is less than 50µl or when using nucleated red blood cell samples, the Cultured Cells Protocol is recommended. If a larger sample size is required, the buffer volume can be scaled proportionately. |
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| IBI Purification Products - Total RNA (Plant) - 96 Well | |
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IBI's Total RNA (Plant) Kit was designed specifically for high-throughput purification of total RNA from plant tissue and cells, using either vacuum or a centrifuge. |
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IBI's Total RNA (Plant) Kit was designed specifically for high-throughput purification of total RNA from plant tissue and cells, using either vacuum or a centrifuge. |
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IBI's Total RNA (Plant) Kit was designed specifically for high-throughput purification of total RNA from plant tissue and cells, using either vacuum or a centrifuge. |
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| IBI Purification Products - Viral DNA Extraction Kits | |
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IBI's Viral DNA Extraction Kit was specifically designed for purification of viral DNA/RNA from cell-free samples; such as, serum, plasma, body fluids, and the supernatant of viral infected cell cultures. These kits are recommended for parallel purification of viral DNA including HBV and CMV, as well as viral RNA including HCV, HIV, and HTLV. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assays. |
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IBI's Viral DNA Extraction Kit was specifically designed for purification of viral DNA/RNA from cell-free samples; such as, serum, plasma, body fluids, and the supernatant of viral infected cell cultures. These kits are recommended for parallel purification of viral DNA including HBV and CMV, as well as viral RNA including HCV, HIV, and HTLV. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assays. |
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IBI's Viral DNA Extraction Kit was specifically designed for purification of viral DNA/RNA from cell-free samples; such as, serum, plasma, body fluids, and the supernatant of viral infected cell cultures. These kits are recommended for parallel purification of viral DNA including HBV and CMV, as well as viral RNA including HCV, HIV, and HTLV. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assays. |
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IBI's Viral DNA Extraction Kit was specifically designed for purification of viral DNA/RNA from cell-free samples; such as, serum, plasma, body fluids, and the supernatant of viral infected cell cultures. These kits are recommended for parallel purification of viral DNA including HBV and CMV, as well as viral RNA including HCV, HIV, and HTLV. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assays. |
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| IBI Purification Products - IBI Nu-Clean Spin Columns | |
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Maximum sample volume: 100ul Click on "details" for full protocol. |
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Maximum sample volume: 100ul Click on "details" for full protocol. |
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Maximum sample volume: 100ul Click on "details" for full protocol. |
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Maximum sample volume: 100ul Click on "details" for full protocol. |
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Maximum sample volume: 100ul Click on "details" for full protocol. |
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| IBI Purification Products - IBI Select-D Spin Columns | |
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Maximum sample volume: 20ul Click on "details" for full protocol. |
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Maximum sample volume: 20ul Click on "details" for full protocol. |
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Maximum sample volume: 50ul Click on "details" for full protocol. |
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Maximum sample volume: 50ul Click on "details" for full protocol. |
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Maximum sample volume: 50ul Click on "details" for full protocol. |
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Maximum sample volume: 100ul Click on "details" for full protocol. |
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Maximum sample volume: 100ul Click on "details" for full protocol. |
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Maximum sample volume: 100ul Click on "details" for full protocol. |
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Maximum sample volume: 100ul Click on "details" for full protocol. |
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